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Rozalyonok T., Kemerovo Institute of Food Science and Technology (University), Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation, rozalyonok@yandex.ru

Sidorin Yu., Kemerovo Institute of Food Science and Technology (University), Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation

Год 2016 Номер журнала 2 DOI 10.21603/2500-1418-2016-1-2-85-91
Аннотация Currently, the problem of micro-organisms resistance to traditional antibiotics, which represents a serious threat to human health, is exposed to close attention. Therefore, the development of alternative antimicrobial agents, including on the basis of silver nanoparticles today becomes relevant. Substantiation of effectiveness of the cluster silver (1-10 nm) in comparison with larger silver nanoparticles is resulted. During the study of domestic and foreign experience of using a cluster of silver, basic mechanisms of its antimicrobial action were analyzed that it may have on organisms. The purpose was to comparatively study the antimicrobial activity of a cluster silver with respect to various microorganisms, including establishment of the minimum inhibitory silver concentration for the following strains: Escherichia coli, Bacillus subtilis, Candida albicans, Aspergillus niger. The effect of various concentrations of silver clusters (from 0 to 400 ug/ml) contained in a liquid medium, on survival of cultured cells was studied. Using the method of serial dilutions, the difference in effects on silver clusters on growth and reproduction of the following microorganisms was established: bacteria (with a different structure of cell walls: gram-positive-thick-walled, capable to form endospores and gram-negative - thin- walled) and micromycetes (yeast and hyphal). An in vitro study of antimicrobial activity of the cluster silver colloidal solution taken at various concentrations and at various exposure times was carried out. Minimum inhibitory concentrations of cluster silver colloidal solution for studied bacteria were determined: opportunistic pathogenic bacterium (Escherichia coli and Bacillus subtilis) and micromycetes (Candida albicans and Aspergillus niger).
Ключевые слова Cluster silver, nanosilver, ions, nanoparticles, antimicrobial activity, MIC
Информация о статье Дата поступления 1 мая 2016 года
Дата принятия в печать 16 июня 2016 года
Дата онлайн-размещения 30 декабря 2016 года
Выходные данные статьи Rozalyonok T., Sidorin Yu. A COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SILVER CLUSTERS AGAINST VARIOUS MICROORGANISMS. Science Evolution, 2016, vol. 1, no. 2, pp. 85-91. doi:10.21603/2500–1418–2016–1–2–85–91.
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Последний выпуск: Science Evolution, Vol. 2, no. 1, 2017